About this paper
Design of experiment (DOE) was utilized to optimize a peptide microarray to test autoantibodies in serum against various components of the myelin sheath. Incorporating the ideal assay conditions from a single DOE experiment versus multiple sequential experiments resulted in a reproducible serum assay with low variability, low nonspecific background, and a high level of distinction between multiple sclerosis (MS) and normal samples. A minimum set of peptides was found that achieved a sensitivity of 90 percent. Autoantibodies against each individual peptide were elevated in at least 35 percent of the individual MS patients tested.
SAS is the leader in analytics. Through innovative analytics, business intelligence and data management software and services, SAS helps customers at more than 83,000 sites make better decisions faster. Since 1976, SAS has been giving customers around the world THE POWER TO KNOW®.