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Analysis of alternative splicing in expressed genes is of growing interest to researchers. While powerful, the standard 3’-biased expression microarrays do not provide a full picture of the molecular events that may be involved in disease. Apparent increases or decreases in summarized transcript levels may be due to alternative exon splicing or, conversely, differences in transcript levels due to alternative splicing may not even be detectable in a 3’-biased assay. Affymetrix microarray platforms can measure exon-level expression levels in humans or rats, and other exon-level assays are also available. The workflow described in this white paper can be generalized for any exon analysis where there is annotation available for grouping probes or sequences at the transcript and exon level.



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